膽堿及其代謝產(chǎn)物在細胞膜的結(jié)構(gòu)完整性和信號傳導以及膽堿能神經(jīng)傳遞（乙酰膽堿合成）中起著重要作用。它是通過其代謝產(chǎn)物基甘氨酸參與S-腺苷甲硫氨酸合成途徑而獲得甲基的主要來源。膽堿缺乏癥可能導致肝臟病癥，動脈粥樣硬化以及可能的神經(jīng)系統(tǒng)病癥。盡管膽堿在神經(jīng)系統(tǒng)中作為乙酰膽堿和膜磷脂酰膽堿的前體具有重要作用，但膽堿在精神病中的作用尚未得到研究。該Amplite 膽堿定量試劑盒提供了靈敏的膽堿定量方法之一。該試劑盒使用Amplite Red量化膽堿的濃度，這與膽堿氧化酶介導的酶偶聯(lián)反應(yīng)中過氧化氫的產(chǎn)生有關(guān)。膽堿的量與在酶偶聯(lián)反應(yīng)循環(huán)中形成的過氧化氫的濃度成比例。在存在過氧化物酶的情況下，Amplite Red的熒光強度與過氧化氫的形成成比例，該過氧化氫轉(zhuǎn)化為膽堿的濃度。該測定可以容易地用熒光酶標儀讀取，或者也可以使用光吸收酶標儀在?570 nm處讀取測定值。百螢生物是AAT Bioquest的中國代理商，為您提供的Amplite 膽堿定量試劑盒。

參考文獻

Structural snapshots of the catalytic cycle of the phosphodiesterase Autotaxin
Authors: Hausmann, Jens and Keune, Willem-Jan and Ederveen, Agnes L Hipgrave and van Zeijl, Leonie and Joosten, Robbie P and Perrakis, Anastassis
Journal: Journal of structural biology (2016)

Real-time multiplex PCR assay for genotyping of three apolipoprotein E alleles and two choline acetyltransferase alleles with three hybridization probes
Authors: Park HD, Park KU, Kim KW, Song J, Chang HE, Heo SR, Lee HJ, Kim JQ.
Journal: Clin Chem Lab Med (2007): 346

[Methyl-3H]-choline incorporation into MCF-7 cells: correlation with proliferation, choline kinase and phospholipase D assay
Authors: Al-Saeedi F, Smith T, Welch A.
Journal: Anticancer Res (2007): 901

A simple and rapid radiochemical choline acetyltransferase (ChAT) assay screening test
Authors: Shiba K, Ogawa K, Kinuya S, Yajima K, Mori H.
Journal: J Neurosci Methods (2006): 98

Rapid high-throughput detection of peroxide with an acridinium-9-carboxamide: a homogeneous chemiluminescent assay for plasma choline
Authors: Adamczyk M, Brashear RJ, Mattingly PG.
Journal: Bioorg Med Chem Lett (2006): 2407