腺苷3'，5'環(huán)一磷酸（cAMP）是細(xì)胞內(nèi)信號(hào)轉(zhuǎn)導(dǎo)的二信使。檢測(cè)cAMP的水平是篩選GPCR激動(dòng)劑和拮抗劑的常用方法之一。 Screen Quest 比色ELISA cAMP分析試劑盒基于HRP標(biāo)記的cAMP與未標(biāo)記的cAMP之間的競(jìng)爭(zhēng)。通過(guò)未標(biāo)記的游離cAMP將HRP-cAMP從HRP-cAMP /抗cAMP抗體復(fù)合物中置換出來(lái)。在不存在cAMP的情況下，HRP-cAMP偶聯(lián)物僅與抗cAMP抗體結(jié)合。然而，測(cè)試樣品中未標(biāo)記的游離cAMP與HRP-cAMP抗體偶聯(lián)物競(jìng)爭(zhēng)抗cAMP抗體，因此抑制了HRP-cAMP與抗cAMP抗體的結(jié)合。我們的Screen Quest 比色ELISA cAMP分析試劑盒提供了一種靈敏的方法，用于在生化或基于細(xì)胞的分析系統(tǒng)中檢測(cè)腺苷酸環(huán)化酶的活性。與其他ELISA cAMP分析試劑盒相比，我們的試劑盒省去了繁瑣的乙酰化步驟。該試劑盒使用Amplite Green作為比色底物來(lái)定量HRP活性。該測(cè)定可以使用96孔或384孔微孔板進(jìn)行。百螢生物是AAT Bioquest的中國(guó)代理商，為您提供的Screen Quest cAMP檢測(cè)試劑盒。

參考文獻(xiàn)

A cardiac mitochondrial cAMP signaling pathway regulates calcium accumulation, permeability transition and cell death
Authors: Wang Z, Liu D, Varin A, Nicolas V, Courilleau D, Mateo P, Caubere C, Rouet P, Gomez AM, V and ecasteele G, Fischmeister R, Brenner C.
Journal: Cell Death Dis (2016): e2198

Activation of P2X7 and P2Y11 purinergic receptors inhibits migration and normalizes tumor-derived endothelial cells via cAMP signaling
Authors: Avanzato, D and Genova, T and Pla, A Fiorio and Bernardini, M and Bianco, S and Bussolati, B and Mancardi, D and Giraudo, E and Maione, F and Cassoni, P and others
Journal: Scientific Reports (2016)

Changes in the Arabidopsis thaliana Proteome Implicate cAMP in Biotic and Abiotic Stress Responses and Changes in Energy Metabolism
Authors: Alqurashi M, Gehring C, Marondedze C.
Journal: Int J Mol Sci (2016): 852

Odor-induced cAMP production in Drosophila melanogaster olfactory sensory neurons
Authors: Miazzi F, Hansson BS, Wicher D.
Journal: J Exp Biol (2016): 1798

Role of the cAMP Pathway in Glucose and Lipid Metabolism
Authors: Ravnskjaer K, Madiraju A, Montminy M.
Journal: Handb Exp Pharmacol (2016): 29