產(chǎn)品基本信息
產(chǎn)品名稱(chēng)：鈣離子熒光探針腔腸素 n
儲(chǔ)存條件：-15℃避光防潮
保質(zhì)期：12個(gè)月
產(chǎn)品物理化學(xué)光譜特性
分子量：457.52
溶劑：乙醇
激發(fā)波長(zhǎng)（nm）：431
發(fā)射波長(zhǎng)（nm）：468
鈣離子熒光探針腔腸素 n是美國(guó)AAT Bioquest生產(chǎn)的用于標(biāo)記鈣離子的熒光探針。鈣的測(cè)量對(duì)于許多生物學(xué)研究至關(guān)重要。水母發(fā)光蛋白復(fù)合物包含22kD的apoaequorin蛋白，分子氧和發(fā)光體coelenterazine。當(dāng)三個(gè)Ca2+離子與該復(fù)合物結(jié)合時(shí)，腔腸素被氧化成腔腸酰胺，伴隨著二氧化碳和藍(lán)光的釋放。水母發(fā)光蛋白的生物發(fā)光對(duì)Ca2+濃度的近似三次冪依賴(lài)性允許測(cè)量Ca2+濃度，檢測(cè)范圍為~0.1μM至>100μM。與熒光Ca2+指示劑不同，可以在不照射樣品的情況下檢測(cè)Ca2+結(jié)合的水母發(fā)光蛋白，從而除自發(fā)熒光的干擾。天然腔腸素及其衍生物賦予水母發(fā)光蛋白復(fù)合物不同的Ca2+親和力和光譜特性。據(jù)報(bào)道，用腔腸素hcp重構(gòu)的重組脫輔基水母蛋白具有總體發(fā)光，具有高量子產(chǎn)率和快速響應(yīng)時(shí)間。然而，來(lái)自腔腸素類(lèi)似物的水母發(fā)光蛋白的細(xì)胞內(nèi)重建可能相對(duì)較慢。含有cp，f或h形式的腔腸素的水母發(fā)光蛋白表現(xiàn)出比用天然腔腸素重構(gòu)的脫輔基水母發(fā)光蛋白強(qiáng)10-20倍的發(fā)光。百螢生物是AAT Bioquest的中國(guó)代理商，為您提供的鈣離子熒光探針腔腸素 n。
參考文獻(xiàn) Molecular characterization of neuropeptide elevenin and two elevenin receptors, IsElevR1 and IsElevR2, from the blacklegged tick, Ixodes scapularis Authors: Donghun Kim, Ladislav Simo, Yoonseong Park Journal: Insect Biochemistry and Molecular Biology (2018) Bioluminescence Resonance Energy Transfer (BRET) Assay for Determination of Molecular Interactions in Living Cells Authors: Kaleeckal G Harikumar, Yan Yan, Ting-Hai Xu, Karsten Melcher, H Eric Xu, Laurence J Miller Journal: (2017) Intracellular Ca2+ is important for flagellin-triggered defense in Arabidopsis and involves inositol polyphosphate signaling Authors: Yi Ma, Yichen Zhao, Gerald A Berkowitz Journal: Journal of Experimental Botany (2017) Orchestration of salivary secretion mediated by two different dopamine receptors in the blacklegged tick Ixodes scapularis Authors: Donghun Kim, Ladislav Simo, Yoonseong Park Journal: Journal of Experimental Biology (2014): 3656--3663
參考文獻(xiàn)
Molecular characterization of neuropeptide elevenin and two elevenin receptors, IsElevR1 and IsElevR2, from the blacklegged tick, Ixodes scapularis Authors: Donghun Kim, Ladislav Simo, Yoonseong Park Journal: Insect Biochemistry and Molecular Biology (2018)
Bioluminescence Resonance Energy Transfer (BRET) Assay for Determination of Molecular Interactions in Living Cells Authors: Kaleeckal G Harikumar, Yan Yan, Ting-Hai Xu, Karsten Melcher, H Eric Xu, Laurence J Miller Journal: (2017)
Intracellular Ca2+ is important for flagellin-triggered defense in Arabidopsis and involves inositol polyphosphate signaling Authors: Yi Ma, Yichen Zhao, Gerald A Berkowitz Journal: Journal of Experimental Botany (2017)
Orchestration of salivary secretion mediated by two different dopamine receptors in the blacklegged tick Ixodes scapularis Authors: Donghun Kim, Ladislav Simo, Yoonseong Park Journal: Journal of Experimental Biology (2014): 3656--3663